Hotspot

ndhE

SSC · 118645-118950 · 306 bp

Back to Rotheca

Pi 0.475

MarkerSeek score 75.50

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 306
variable_sites: 141
pi: 0.475
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	TCTTTCTGGGATCGGGTCTT	TACATCCGTCCCAGGGTATC	50.0	55.0	58.0	58.0	1353 bp	0.5	59.6
ndhE_p2	ACGTCCTGGTATGGGATCAT	TACATCCGTCCCAGGGTATC	50.0	55.0	57.9	58.0	1039 bp	0.5	59.1
ndhE_p3	TCTTTCTGGGATCGGGTCTT	TGAGCACGGATTTTGTCGAT	50.0	45.0	58.0	57.9	1558 bp	0.5	58.9
ndhE_p4	TCTTTCTGGGATCGGGTCTT	GACCCATACTGCGAGTTGTT	50.0	50.0	58.0	57.9	1485 bp	0.5	58.9
ndhE_p5	TCTTTCTGGGATCGGGTCTT	CTTATTCGCATCAAACCCGC	50.0	50.0	58.0	57.9	1070 bp	0.5	58.8