Hotspot

ndhD

SSC · 119566-121068 · 1503 bp

Back to Rotheca

Pi 0.473

MarkerSeek score 82.80

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 653
pi: 0.473
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GATACCCTGGGACGGATGTA	TGAGTCGTTTTTCCTTGGGG	55.0	50.0	58.0	58.0	2428 bp	0.5	59.8
ndhD_p2	GATACCCTGGGACGGATGTA	TTGGAGTTGGGACCCAAAAG	55.0	50.0	58.0	57.9	2215 bp	0.5	59.3
ndhD_p3	GATACCCTGGGACGGATGTA	GGAGTTGGGACCCAAAAGAG	55.0	55.0	58.0	58.1	2213 bp	0.5	59.2
ndhD_p4	GATACCCTGGGACGGATGTA	GGGACCCAAAAGAGACTTGG	55.0	55.0	58.0	58.1	2207 bp	0.5	59.2
ndhD_p5	ATCGACAAAATCCGTGCTCA	TGAGTCGTTTTTCCTTGGGG	45.0	50.0	57.9	58.0	2223 bp	0.5	59.1