Hotspot

rpl32-ndhF

SSC · 122937-123446 · 510 bp

Back to Rotheca

Pi 0.45

MarkerSeek score 77.90

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 510
variable_sites: 186
pi: 0.45
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	TAGGAGATGATATGCCGCCA	GCGCCCGCAAATAATAGAAC	50.0	50.0	58.1	58.0	1828 bp	0.5	59.2
rpl32-ndhF_p2	TAGGAGATGATATGCCGCCA	TGTGGCTGTCGGAAAAAGAA	50.0	45.0	58.1	58.0	1217 bp	0.5	59.1
rpl32-ndhF_p3	GTTTAGTTGTTTGTCGCGCA	GCGCCCGCAAATAATAGAAC	45.0	50.0	57.9	58.0	1380 bp	0.5	59.0
rpl32-ndhF_p4	CACCCCTTCCCTTTCCAAAA	GCGCCCGCAAATAATAGAAC	50.0	50.0	57.9	58.0	1294 bp	0.5	59.0
rpl32-ndhF_p5	GTTTAGTTGTTTGTCGCGCA	TGTGGCTGTCGGAAAAAGAA	45.0	45.0	57.9	58.0	769 bp	0.5	58.9