Hotspot

trnL-UAG-rpl32

SSC · 122421-122759 · 339 bp

Back to Rotheca

Pi 0.46
MarkerSeek score 77.20
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
339
variable_sites
143
pi
0.46
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 ATGCCCCTTTCGGATGAATC CAAAGGCAAAATCCACGCAT 50.0 45.0 58.0 57.9 1363 bp 0.5 59.2
trnL-UAG-rpl32_p2 GCCCCTTTCGGATGAATCAT CAAAGGCAAAATCCACGCAT 50.0 45.0 58.0 57.9 1361 bp 0.5 59.2
trnL-UAG-rpl32_p3 CACATAAAAGCGCGGCATAG CAAAGGCAAAATCCACGCAT 50.0 45.0 58.0 57.9 1686 bp 0.5 59.2
trnL-UAG-rpl32_p4 ATGCCCCTTTCGGATGAATC TGCGCGACAAACAACTAAAC 50.0 45.0 58.0 57.9 674 bp 0.5 59.1
trnL-UAG-rpl32_p5 GCCCCTTTCGGATGAATCAT TGCGCGACAAACAACTAAAC 50.0 45.0 58.0 57.9 672 bp 0.5 59.1