Hotspot

ndhI-ndhG

SSC · 117562-117913 · 352 bp

Back to Rotheca

Pi 0.517

MarkerSeek score 77.40

Primer available yes

Label ndhI-ndhG

Feature Metadata

label_name: ndhI-ndhG
feature_type: igs
region: SSC
length_bp: 352
variable_sites: 166
pi: 0.517
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhG_p1	TGATTACCTTGTCCCATGCG	AAGACCCGATCCCAGAAAGA	50.0	50.0	58.0	58.0	837 bp	0.5	59.3
ndhI-ndhG_p2	TGCCGGCATCAATAATGGAA	AAGACCCGATCCCAGAAAGA	45.0	50.0	57.9	58.0	483 bp	0.5	59.1
ndhI-ndhG_p3	TTGCCGGCATCAATAATGGA	AAGACCCGATCCCAGAAAGA	45.0	50.0	57.9	58.0	484 bp	0.5	59.1
ndhI-ndhG_p4	ACATCAGAACGTTTCCGAGG	AAGACCCGATCCCAGAAAGA	50.0	50.0	57.9	58.0	772 bp	0.5	59.0
ndhI-ndhG_p5	CATCAGAACGTTTCCGAGGT	AAGACCCGATCCCAGAAAGA	50.0	50.0	57.9	58.0	771 bp	0.5	59.0