Hotspot

ycf1-rps15

SSC · 112923-113328 · 406 bp

Back to Rotheca

Pi 0.485

MarkerSeek score 76.00

Primer available yes

Label ycf1-rps15

Feature Metadata

label_name: ycf1-rps15
feature_type: igs
region: SSC
length_bp: 406
variable_sites: 162
pi: 0.485
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps15_p1	CGGAAAGAACAGTAGCCAGG	CAGCAGTCGTTGACGTTTTC	55.0	50.0	58.3	58.0	782 bp	0.5	57.8
ycf1-rps15_p2	GAACCCATTCGTCTGTCTGG	CAGCAGTCGTTGACGTTTTC	55.0	50.0	58.3	58.0	1093 bp	0.5	57.8
ycf1-rps15_p3	CGGAAAGAACAGTAGCCAGG	TCAAATGGTATCGCGCTTCA	55.0	45.0	58.3	58.0	893 bp	0.5	57.7
ycf1-rps15_p4	GAACCCATTCGTCTGTCTGG	TCAAATGGTATCGCGCTTCA	55.0	45.0	58.3	58.0	1204 bp	0.5	57.7
ycf1-rps15_p5	CGGAAAGAACAGTAGCCAGG	AGTCGTTGACGTTTTCCCAA	55.0	45.0	58.3	58.0	778 bp	0.5	57.6