primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ycf1	1	CATTTATCTCCGACCGGGTG	GCGGGAGTTTGGTGGATTTA	20	20	55.0	50.0	58.1	58.2	3.3	0.0	0.305156	111619	111738	588-588	0.500000	57.6
ycf1_p2	ycf1	2	CATTTATCTCCGACCGGGTG	AGATCCTTTGCGGGAGTTTG	20	20	55.0	50.0	58.1	58.2	3.3	0.0	0.307750	111619	111738	597-597	0.500000	57.5
ycf1_p3	ycf1	3	CATTTATCTCCGACCGGGTG	GATCTTCCCACCACCTTGTG	20	20	55.0	55.0	58.1	58.2	3.3	0.0	0.307750	111619	111738	868-868	0.500000	57.5
ycf1_p4	ycf1	4	CATTTATCTCCGACCGGGTG	CCAGACAGACGAATGGGTTC	20	20	55.0	55.0	58.1	58.3	3.3	0.0	0.407282	111619	111738	1223-1223	0.500000	56.7
ycf1_p5	ycf1	5	TCCACCCCTTTCTTAAGCCT	GCGGGAGTTTGGTGGATTTA	20	20	50.0	50.0	58.2	58.2	0.0	0.0	0.415821	111619	111738	725-725	0.500000	56.7
ycf1-rps15_p1	ycf1-rps15	1	CGGAAAGAACAGTAGCCAGG	CAGCAGTCGTTGACGTTTTC	20	20	55.0	50.0	58.3	58.0	0.0	3.2	0.278335	112923	113328	782-782	0.500000	57.8
ycf1-rps15_p2	ycf1-rps15	2	GAACCCATTCGTCTGTCTGG	CAGCAGTCGTTGACGTTTTC	20	20	55.0	50.0	58.3	58.0	0.0	3.2	0.278335	112923	113328	1093-1093	0.500000	57.8
ycf1-rps15_p3	ycf1-rps15	3	CGGAAAGAACAGTAGCCAGG	TCAAATGGTATCGCGCTTCA	20	20	55.0	45.0	58.3	58.0	0.0	5.6	0.290285	112923	113328	893-893	0.500000	57.7
ycf1-rps15_p4	ycf1-rps15	4	GAACCCATTCGTCTGTCTGG	TCAAATGGTATCGCGCTTCA	20	20	55.0	45.0	58.3	58.0	0.0	5.6	0.290285	112923	113328	1204-1204	0.500000	57.7
ycf1-rps15_p5	ycf1-rps15	5	CGGAAAGAACAGTAGCCAGG	AGTCGTTGACGTTTTCCCAA	20	20	55.0	45.0	58.3	58.0	0.0	3.2	0.300150	112923	113328	778-778	0.500000	57.6
rps15-ndhH_p1	rps15-ndhH	1	GAAAACGTCAACGACTGCTG	TCCGGAAGCTCGTAACATTG	20	20	50.0	50.0	58.0	58.0	9.9	0.0	0.009771	113602	113705	889-889	0.500000	59.9
rps15-ndhH_p2	rps15-ndhH	2	TTGGGAAAACGTCAACGACT	TCCGGAAGCTCGTAACATTG	20	20	45.0	50.0	58.0	58.0	9.9	0.0	0.031586	113602	113705	893-893	0.500000	59.7
rps15-ndhH_p3	rps15-ndhH	3	GAAAACGTCAACGACTGCTG	CGTAACATTGGCCCCGATAA	20	20	50.0	50.0	58.0	58.0	9.9	0.0	0.048892	113602	113705	879-879	0.500000	59.6
rps15-ndhH_p4	rps15-ndhH	4	TTGGGAAAACGTCAACGACT	CGTAACATTGGCCCCGATAA	20	20	45.0	50.0	58.0	58.0	9.9	0.0	0.070707	113602	113705	883-883	0.500000	59.4
rps15-ndhH_p5	rps15-ndhH	5	GAAAACGTCAACGACTGCTG	ATCAGTCGAAGAACACCGTG	20	20	50.0	50.0	58.0	57.9	9.9	0.0	0.071181	113602	113705	291-291	0.500000	59.4
ndhH_p1	ndhH	1	GAAAACGTCAACGACTGCTG	CGTTGTTGTATCCCTGCAGA	20	20	50.0	50.0	58.0	57.9	9.9	17.4	0.098495	113706	114887	1570-1570	0.500000	59.2
ndhH_p2	ndhH	2	TGAAGCGCGATACCATTTGA	CGTTGTTGTATCCCTGCAGA	20	20	45.0	50.0	58.0	57.9	12.1	17.4	0.110444	113706	114887	1459-1459	0.500000	59.1
ndhH_p3	ndhH	3	GAAAACGTCAACGACTGCTG	AGCAGCCCTTAATCCACCTA	20	20	50.0	50.0	58.0	58.1	9.9	0.0	0.111873	113706	114887	1883-1883	0.500000	59.1
ndhH_p4	ndhH	4	TTGGGAAAACGTCAACGACT	CGTTGTTGTATCCCTGCAGA	20	20	45.0	50.0	58.0	57.9	9.9	17.4	0.120310	113706	114887	1574-1574	0.500000	59.0
ndhH_p5	ndhH	5	TGCATGGAAGAATGACTCGG	CGTTGTTGTATCCCTGCAGA	20	20	50.0	50.0	58.0	57.9	0.0	17.4	0.121726	113706	114887	1394-1394	0.500000	59.0
ndhA_p1	ndhA	1	GCCCGGTTTTATCAACTTGC	AGCCACGATTGATTCTCACC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.039039	114889	116974	2905-2905	0.500000	59.7
ndhA_p2	ndhA	2	GCCCGGTTTTATCAACTTGC	TCCATTATTGATGCCGGCAA	20	20	50.0	45.0	58.0	57.9	0.0	36.3	0.073634	114889	116974	2734-2734	0.500000	59.4
ndhA_p3	ndhA	3	GCCCGGTTTTATCAACTTGC	TTCCATTATTGATGCCGGCA	20	20	50.0	45.0	58.0	57.9	0.0	34.8	0.073634	114889	116974	2735-2735	0.500000	59.4
ndhA_p4	ndhA	4	CAATGTTACGAGCTTCCGGA	TGGCAGCTCGTATTGTTTGT	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.113706	114889	116974	2743-2743	0.500000	59.1
ndhA_p5	ndhA	5	CAGGCTCTGGAAGGAATTCC	TCCATTATTGATGCCGGCAA	20	20	55.0	45.0	58.0	57.9	17.3	36.3	0.114322	114889	116974	2973-2973	0.500000	59.1
ndhI_p1	ndhI	1	CTCTAGCCGAGTGTGAGAGA	AGCCACGATTGATTCTCACC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.061097	117055	117561	1131-1131	0.500000	59.5
ndhI_p2	ndhI	2	TCTCTAGCCGAGTGTGAGAG	AGCCACGATTGATTCTCACC	20	20	55.0	50.0	58.0	58.0	1.1	0.0	0.061097	117055	117561	1132-1132	0.500000	59.5
ndhI_p3	ndhI	3	CTCTAGCCGAGTGTGAGAGA	AAGACCCGATCCCAGAAAGA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.078637	117055	117561	1424-1424	0.500000	59.4
ndhI_p4	ndhI	4	TCTCTAGCCGAGTGTGAGAG	AAGACCCGATCCCAGAAAGA	20	20	55.0	50.0	58.0	58.0	1.1	0.0	0.078637	117055	117561	1425-1425	0.500000	59.4
ndhI_p5	ndhI	5	CTCTAGCCGAGTGTGAGAGA	ATGATCCCATACCAGGACGT	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.134412	117055	117561	1738-1738	0.500000	58.9
ndhI-ndhG_p1	ndhI-ndhG	1	TGATTACCTTGTCCCATGCG	AAGACCCGATCCCAGAAAGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.084336	117562	117913	837-837	0.500000	59.3
ndhI-ndhG_p2	ndhI-ndhG	2	TGCCGGCATCAATAATGGAA	AAGACCCGATCCCAGAAAGA	20	20	45.0	50.0	57.9	58.0	29.0	0.0	0.111812	117562	117913	483-483	0.500000	59.1
ndhI-ndhG_p3	ndhI-ndhG	3	TTGCCGGCATCAATAATGGA	AAGACCCGATCCCAGAAAGA	20	20	45.0	50.0	57.9	58.0	33.2	0.0	0.111812	117562	117913	484-484	0.500000	59.1
ndhI-ndhG_p4	ndhI-ndhG	4	ACATCAGAACGTTTCCGAGG	AAGACCCGATCCCAGAAAGA	20	20	50.0	50.0	57.9	58.0	0.5	0.0	0.127665	117562	117913	772-772	0.500000	59.0
ndhI-ndhG_p5	ndhI-ndhG	5	CATCAGAACGTTTCCGAGGT	AAGACCCGATCCCAGAAAGA	20	20	50.0	50.0	57.9	58.0	0.5	0.0	0.127665	117562	117913	771-771	0.500000	59.0
ndhG_p1	ndhG	1	TGCCGGCATCAATAATGGAA	CTTATTCGCATCAAACCCGC	20	20	45.0	50.0	57.9	57.9	29.0	0.0	0.169756	117914	118444	1533-1533	0.500000	58.6
ndhG_p2	ndhG	2	TTGCCGGCATCAATAATGGA	CTTATTCGCATCAAACCCGC	20	20	45.0	50.0	57.9	57.9	33.2	0.0	0.169756	117914	118444	1534-1534	0.500000	58.6
ndhG_p3	ndhG	3	ACATCAGAACGTTTCCGAGG	CTTATTCGCATCAAACCCGC	20	20	50.0	50.0	57.9	57.9	0.5	0.0	0.185609	117914	118444	1822-1822	0.500000	58.5
ndhG_p4	ndhG	4	CATCAGAACGTTTCCGAGGT	CTTATTCGCATCAAACCCGC	20	20	50.0	50.0	57.9	57.9	0.5	0.0	0.185609	117914	118444	1821-1821	0.500000	58.5
ndhG_p5	ndhG	5	TGCCGGCATCAATAATGGAA	AGACGAATCAATGGGCGATC	20	20	45.0	50.0	57.9	58.1	29.0	0.0	0.193353	117914	118444	1635-1635	0.500000	58.5
ndhE_p1	ndhE	1	TCTTTCTGGGATCGGGTCTT	TACATCCGTCCCAGGGTATC	20	20	50.0	55.0	58.0	58.0	0.0	5.0	0.052749	118645	118950	1353-1353	0.500000	59.6
ndhE_p2	ndhE	2	ACGTCCTGGTATGGGATCAT	TACATCCGTCCCAGGGTATC	20	20	50.0	55.0	57.9	58.0	0.0	5.0	0.108524	118645	118950	1039-1039	0.500000	59.1
ndhE_p3	ndhE	3	TCTTTCTGGGATCGGGTCTT	TGAGCACGGATTTTGTCGAT	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.140685	118645	118950	1558-1558	0.500000	58.9
ndhE_p4	ndhE	4	TCTTTCTGGGATCGGGTCTT	GACCCATACTGCGAGTTGTT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.142141	118645	118950	1485-1485	0.500000	58.9
ndhE_p5	ndhE	5	TCTTTCTGGGATCGGGTCTT	CTTATTCGCATCAAACCCGC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.152701	118645	118950	1070-1070	0.500000	58.8
ndhE-psaC_p1	ndhE-psaC	1	ACGTCCTGGTATGGGATCAT	TACATCCGTCCCAGGGTATC	20	20	50.0	55.0	57.9	58.0	0.0	5.0	0.108524	118951	119200	1039-1039	0.500000	59.1
ndhE-psaC_p2	ndhE-psaC	2	ACGTCCTGGTATGGGATCAT	TGAGCACGGATTTTGTCGAT	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.196460	118951	119200	1244-1244	0.500000	58.4
ndhE-psaC_p3	ndhE-psaC	3	ACGTCCTGGTATGGGATCAT	GACCCATACTGCGAGTTGTT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.197916	118951	119200	1171-1171	0.500000	58.4
ndhE-psaC_p4	ndhE-psaC	4	ACGTCCTGGTATGGGATCAT	TCCGTCCCAGGGTATCATTT	20	20	50.0	50.0	57.9	58.1	0.0	5.0	0.215356	118951	119200	1035-1035	0.500000	58.3
ndhE-psaC_p5	ndhE-psaC	5	ACGTCCTGGTATGGGATCAT	ATCCGTCCCAGGGTATCATT	20	20	50.0	50.0	57.9	57.9	0.0	5.0	0.221557	118951	119200	1036-1036	0.500000	58.2
psaC_p1	psaC	1	GCGGGTTTGATGCGAATAAG	TGAGCACGGATTTTGTCGAT	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.198629	119201	119446	508-508	0.500000	58.4
psaC_p2	psaC	2	GATCGCCCATTGATTCGTCT	TGAGCACGGATTTTGTCGAT	20	20	50.0	45.0	58.1	57.9	0.0	0.0	0.222226	119201	119446	406-406	0.500000	58.2
psaC_p3	psaC	3	TCTGATCGCCCATTGATTCG	TGAGCACGGATTTTGTCGAT	20	20	50.0	45.0	58.1	57.9	0.0	0.0	0.223532	119201	119446	409-409	0.500000	58.2
psaC_p4	psaC	4	GCGGGTTTGATGCGAATAAG	GAGAATCTCGAGTAACGGGC	20	20	50.0	55.0	57.9	57.9	0.0	13.1	0.227886	119201	119446	899-899	0.500000	58.2
psaC_p5	psaC	5	GCGGGTTTGATGCGAATAAG	TTGAGCACGGATTTTGTCGA	20	20	50.0	45.0	57.9	58.1	0.0	0.0	0.232935	119201	119446	509-509	0.500000	58.1
ndhD_p1	ndhD	1	GATACCCTGGGACGGATGTA	TGAGTCGTTTTTCCTTGGGG	20	20	55.0	50.0	58.0	58.0	22.2	0.0	0.029711	119566	121068	2428-2428	0.500000	59.8
ndhD_p2	ndhD	2	GATACCCTGGGACGGATGTA	TTGGAGTTGGGACCCAAAAG	20	20	55.0	50.0	58.0	57.9	22.2	27.9	0.086024	119566	121068	2215-2215	0.500000	59.3
ndhD_p3	ndhD	3	GATACCCTGGGACGGATGTA	GGAGTTGGGACCCAAAAGAG	20	20	55.0	55.0	58.0	58.1	22.2	27.9	0.094313	119566	121068	2213-2213	0.500000	59.2
ndhD_p4	ndhD	4	GATACCCTGGGACGGATGTA	GGGACCCAAAAGAGACTTGG	20	20	55.0	55.0	58.0	58.1	22.2	5.3	0.094313	119566	121068	2207-2207	0.500000	59.2
ndhD_p5	ndhD	5	ATCGACAAAATCCGTGCTCA	TGAGTCGTTTTTCCTTGGGG	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.117647	119566	121068	2223-2223	0.500000	59.1
ccsA_p1	ccsA	1	AACGGGGCCCTTTTACAAAT	TGGCGGCATATCATCTCCTA	20	20	45.0	50.0	58.0	58.1	24.4	8.0	0.081164	121285	122256	1799-1799	0.500000	59.4
ccsA_p2	ccsA	2	AACGGGGCCCTTTTACAAAT	TGCGCGACAAACAACTAAAC	20	20	45.0	45.0	58.0	57.9	24.4	12.1	0.112327	121285	122256	2247-2247	0.500000	59.1
ccsA_p3	ccsA	3	AACGGGGCCCTTTTACAAAT	TTTTGGAAAGGGAAGGGGTG	20	20	45.0	50.0	58.0	57.9	24.4	0.0	0.113959	121285	122256	2333-2333	0.500000	59.1
ccsA_p4	ccsA	4	AACGGGGCCCTTTTACAAAT	TAGACACGCTGCTCTTAGGA	20	20	45.0	50.0	58.0	57.9	24.4	0.0	0.142632	121285	122256	1852-1852	0.500000	58.9
ccsA_p5	ccsA	5	AACGGGGCCCTTTTACAAAT	ACAAGCCGCTATGGTGAAAT	20	20	45.0	45.0	58.0	57.9	24.4	0.0	0.147126	121285	122256	1875-1875	0.500000	58.8
trnL-UAG-rpl32_p1	trnL-UAG-rpl32	1	ATGCCCCTTTCGGATGAATC	CAAAGGCAAAATCCACGCAT	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.096284	122421	122759	1363-1363	0.500000	59.2
trnL-UAG-rpl32_p2	trnL-UAG-rpl32	2	GCCCCTTTCGGATGAATCAT	CAAAGGCAAAATCCACGCAT	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.096284	122421	122759	1361-1361	0.500000	59.2
trnL-UAG-rpl32_p3	trnL-UAG-rpl32	3	CACATAAAAGCGCGGCATAG	CAAAGGCAAAATCCACGCAT	20	20	50.0	45.0	58.0	57.9	8.8	0.0	0.105975	122421	122759	1686-1686	0.500000	59.2
trnL-UAG-rpl32_p4	trnL-UAG-rpl32	4	ATGCCCCTTTCGGATGAATC	TGCGCGACAAACAACTAAAC	20	20	50.0	45.0	58.0	57.9	0.0	12.1	0.107089	122421	122759	674-674	0.500000	59.1
trnL-UAG-rpl32_p5	trnL-UAG-rpl32	5	GCCCCTTTCGGATGAATCAT	TGCGCGACAAACAACTAAAC	20	20	50.0	45.0	58.0	57.9	0.0	12.1	0.107089	122421	122759	672-672	0.500000	59.1
rpl32-ndhF_p1	rpl32-ndhF	1	TAGGAGATGATATGCCGCCA	GCGCCCGCAAATAATAGAAC	20	20	50.0	50.0	58.1	58.0	0.0	7.9	0.095223	122937	123446	1828-1828	0.500000	59.2
rpl32-ndhF_p2	rpl32-ndhF	2	TAGGAGATGATATGCCGCCA	TGTGGCTGTCGGAAAAAGAA	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.108370	122937	123446	1217-1217	0.500000	59.1
rpl32-ndhF_p3	rpl32-ndhF	3	GTTTAGTTGTTTGTCGCGCA	GCGCCCGCAAATAATAGAAC	20	20	45.0	50.0	57.9	58.0	8.7	7.9	0.126386	122937	123446	1380-1380	0.500000	59.0
rpl32-ndhF_p4	rpl32-ndhF	4	CACCCCTTCCCTTTCCAAAA	GCGCCCGCAAATAATAGAAC	20	20	50.0	50.0	57.9	58.0	0.0	7.9	0.128018	122937	123446	1294-1294	0.500000	59.0
rpl32-ndhF_p5	rpl32-ndhF	5	GTTTAGTTGTTTGTCGCGCA	TGTGGCTGTCGGAAAAAGAA	20	20	45.0	45.0	57.9	58.0	8.7	0.0	0.139533	122937	123446	769-769	0.500000	58.9
ndhF_p1	ndhF	1	GTTTAGTTGTTTGTCGCGCA	TAGACCCGTTTCCGAAGAGT	20	20	45.0	50.0	57.9	58.1	8.7	0.0	0.178831	123447	125663	2987-2987	0.500000	58.6
ndhF_p2	ndhF	2	CACCCCTTCCCTTTCCAAAA	TAGACCCGTTTCCGAAGAGT	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.180463	123447	125663	2901-2901	0.500000	58.6
ndhF_p3	ndhF	3	AGGTTCGGTGAATCGGAAAC	ATACCGTCGCCCCTTTTTAC	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.223819	123447	125663	2649-2649	0.500000	58.2
ndhF_p4	ndhF	4	CACCCCTTCCCTTTCCAAAA	AAGGCGGATCCGAACAAAAT	20	20	50.0	45.0	57.9	58.2	0.0	37.6	0.261416	123447	125663	2999-2999	0.500000	57.9
ndhF_p5	ndhF	5	AGGTTCGGTGAATCGGAAAC	TTTAGGCAGAATACCGTCGC	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.264832	123447	125663	2659-2659	0.500000	57.9