Hotspot

ycf1

SSC · 121541-126550 · 5010 bp

Back to Rosularia

Pi 0.0822

MarkerSeek score 71.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5010
variable_sites: 408
pi: 0.0822
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACTTAGGTTGGTTTGCTCGG	GCGAACTAGATGAATGGGGT	50.0	50.0	58.1	57.4	1229 bp	0.5	54.2
ycf1_p2	CTTAGGTTGGTTTGCTCGGT	GCGAACTAGATGAATGGGGT	50.0	50.0	58.1	57.4	1228 bp	0.5	54.2
ycf1_p3	ACTTAGGTTGGTTTGCTCGG	TGGGTTGAGCGAACTAGATG	50.0	50.0	58.1	57.3	1237 bp	0.5	53.8
ycf1_p4	CTTAGGTTGGTTTGCTCGGT	TGGGTTGAGCGAACTAGATG	50.0	50.0	58.1	57.3	1236 bp	0.5	53.8
ycf1_p5	CTTAGGTTGGTTTGCTCGGT	ATTCACGAATCGCCCATTCC	50.0	50.0	58.1	58.7	1166 bp	0.5	53.6