Hotspot

rpl32-trnL-UAG

SSC · 111677-112104 · 428 bp

Back to Rosularia

Pi 0.17

MarkerSeek score 79.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 428
variable_sites: 70
pi: 0.17
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGCAGCGTTAAAAGCCTTTT	TTCGAACCGAGATGCTCAAG	45.0	50.0	57.9	58.0	576-610 bp	1.0	88.9
rpl32-trnL-UAG_p2	GGCAGCGTTAAAAGCCTTTT	TTAGAAGAGAATGTGCCGCC	45.0	50.0	57.9	58.0	603-637 bp	1.0	88.7
rpl32-trnL-UAG_p3	GGCAGCGTTAAAAGCCTTTT	AAGCACTGCTTCCTAAGAGC	45.0	50.0	57.9	57.9	559-593 bp	1.0	88.2
rpl32-trnL-UAG_p4	AAAAGGAAGGGGTATTGGGC	TTCGAACCGAGATGCTCAAG	50.0	50.0	57.8	58.0	593 bp	0.5	58.0
rpl32-trnL-UAG_p5	AAAAGGAAGGGGTATTGGGC	TTAGAAGAGAATGTGCCGCC	50.0	50.0	57.8	58.0	620 bp	0.5	57.8