Hotspot

ndhF-rpl32

SSC · 110928-111517 · 590 bp

Back to Rosularia

Pi 0.118
MarkerSeek score 84.50
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
590
variable_sites
67
pi
0.118
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 AGGTATGATCCATGCATATTGA TGCCAGAGAAGTACGCTTTT 36.4 45.0 54.8 57.5 658-659 bp 1.0 50.4
ndhF-rpl32_p2 AGGTATGATCCATGCATATTGA TTGCCAGAGAAGTACGCTTT 36.4 45.0 54.8 57.5 659-660 bp 1.0 50.4
ndhF-rpl32_p3 AGGTATGATCCATGCATATTGA TTTGCCAGAGAAGTACGCTT 36.4 45.0 54.8 57.5 660-661 bp 1.0 50.4
ndhF-rpl32_p4 AGGTATGATCCATGCATATTGA TTTTGCCAGAGAAGTACGCT 36.4 45.0 54.8 57.5 661-662 bp 1.0 50.4
ndhF-rpl32_p5 AGGTATGATCCATGCATATTGAT TGCCAGAGAAGTACGCTTTT 34.8 45.0 55.4 57.5 658-659 bp 1.0 50.4