Hotspot

rps18-rpl20

LSC · 67123-67361 · 239 bp

Back to Rosularia

Pi 0.0929

MarkerSeek score 81.30

Primer available yes

Label rps18-rpl20

Feature Metadata

label_name: rps18-rpl20
feature_type: igs
region: LSC
length_bp: 239
variable_sites: 21
pi: 0.0929
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-rpl20_p1	CTGAGTCGACTGCTCAAACA	TGAAATATTGGATCCATTGGAGT	50.0	34.8	57.9	55.7	299-311 bp	1.0	48.9
rps18-rpl20_p2	ACTGAGTCGACTGCTCAAAC	TGAAATATTGGATCCATTGGAGT	50.0	34.8	57.9	55.7	300-312 bp	1.0	48.9
rps18-rpl20_p3	TGAGTCGACTGCTCAAACAA	TGAAATATTGGATCCATTGGAGT	45.0	34.8	57.4	55.7	298-310 bp	1.0	48.9
rps18-rpl20_p4	AGAACTGAGTCGACTGCTCA	TGAAATATTGGATCCATTGGAGT	50.0	34.8	58.4	55.7	303-315 bp	1.0	48.8
rps18-rpl20_p5	GAACTGAGTCGACTGCTCAA	TGAAATATTGGATCCATTGGAGT	50.0	34.8	57.6	55.7	302-314 bp	1.0	48.8