Hotspot

trnP-UGG-psaJ

LSC · 65651-65908 · 258 bp

Back to Rosularia

Pi 0.226

MarkerSeek score 76.60

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 258
variable_sites: 53
pi: 0.226
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	CGCTCTGTAGGACTTGAACC	GGCCATGAAACTCCTTTGGA	55.0	50.0	58.0	58.1	1092-1112 bp	1.0	89.6
trnP-UGG-psaJ_p2	CGCTCTGTAGGACTTGAACC	GTCAGCGCATCTGGGAATAA	55.0	50.0	58.0	58.0	672 bp	0.5	59.6
trnP-UGG-psaJ_p3	CGAACTGAACTAAGAGCGCT	GTCAGCGCATCTGGGAATAA	50.0	50.0	58.0	58.0	619 bp	0.5	59.5
trnP-UGG-psaJ_p4	AACGCTTTCGGTAGGATTCC	GTCAGCGCATCTGGGAATAA	50.0	50.0	58.0	58.0	1061 bp	0.5	59.5
trnP-UGG-psaJ_p5	GGAACGCTTTCGGTAGGATT	GTCAGCGCATCTGGGAATAA	50.0	50.0	58.0	58.0	1063 bp	0.5	59.5