Hotspot

psbE-petL

LSC · 63713-64836 · 1124 bp

Back to Rosularia

Pi 0.0799

MarkerSeek score 80.40

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1124
variable_sites: 76
pi: 0.0799
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	CCGAAATAACCTGTTATAGTAAGCA	50.0	36.0	57.8	57.0	1045-1177 bp	1.0	49.5
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	ACCGAAATAACCTGTTATAGTAAGC	50.0	36.0	57.8	57.0	1046-1178 bp	1.0	49.5
psbE-petL_p3	GTTCTCCTGTGCTTCCAGAC	CCGAAATAACCTGTTATAGTAAGCA	55.0	36.0	58.2	57.0	1040-1172 bp	1.0	49.5
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	ACCGAAATAACCTGTTATAGTAAGC	55.0	36.0	58.2	57.0	1041-1173 bp	1.0	49.5
psbE-petL_p5	TCTCCTGTGCTTCCAGACAT	CCGAAATAACCTGTTATAGTAAGCA	50.0	36.0	58.4	57.0	1038-1170 bp	1.0	49.5