Hotspot

trnF-GAA-ndhJ

LSC · 47753-48341 · 589 bp

Back to Rosularia

Pi 0.111

MarkerSeek score 80.80

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 589
variable_sites: 64
pi: 0.111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	CGTTCCCAATGTGCCTATGA	55.0	50.0	58.0	58.0	983-1058 bp	1.0	86.6
trnF-GAA-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	ATCTACGTTCCCAATGTGCC	55.0	50.0	58.0	58.0	988-1063 bp	1.0	86.5
trnF-GAA-ndhJ_p3	GGGATAGCTCAGCTGGTAGA	AAGTCCGAGGATTGGCATTC	55.0	50.0	58.0	58.0	1043-1118 bp	1.0	86.2
trnF-GAA-ndhJ_p4	GGGATAGCTCAGCTGGTAGA	AAAGATGCAGGGTCGTTTGT	55.0	45.0	58.0	58.0	1140-1215 bp	1.0	86.0
trnF-GAA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	ATCCCCGCTTGAAACGTATC	55.0	50.0	58.0	58.1	763 bp	0.5	59.6