Hotspot

trnE-UUC-trnT-GGU

LSC · 31496-32308 · 813 bp

Back to Rosularia

Pi 0.0702

MarkerSeek score 81.50

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 813
variable_sites: 56
pi: 0.0702
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CATAGTACCCTACCCCCAGG	GAACCGATGACTTACGCCTT	60.0	50.0	58.0	58.0	951-964 bp	1.0	85.7
trnE-UUC-trnT-GGU_p2	CATAGTACCCTACCCCCAGG	CCATGGCGTTACTCTACCAC	60.0	55.0	58.0	58.1	930-943 bp	1.0	85.3
trnE-UUC-trnT-GGU_p3	CATAGTACCCTACCCCCAGG	TGGCGTTACTCTACCACTGA	60.0	50.0	58.0	58.1	927-940 bp	1.0	85.2
trnE-UUC-trnT-GGU_p4	CATAGTACCCTACCCCCAGG	CCGATGACTTACGCCTTACC	60.0	55.0	58.0	58.2	948-961 bp	1.0	84.5
trnE-UUC-trnT-GGU_p5	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	916-929 bp	1.0	84.0