Hotspot

psbM-trnD-GUC

LSC · 29900-30907 · 1008 bp

Back to Rosularia

Pi 0.0913

MarkerSeek score 84.00

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1008
variable_sites: 89
pi: 0.0913
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAGTGCAGTAGC	TCAATTGGTAAGAGCACCGC	45.0	50.0	55.8	58.6	1102-1118 bp	1.0	66.6
psbM-trnD-GUC_p2	AGAATGAACAGTGCAGTAGC	AATTGGTAAGAGCACCGCC	45.0	52.6	55.8	58.1	1100-1116 bp	1.0	61.9
psbM-trnD-GUC_p3	AGAATGAACAGTGCAGTAGC	GTTCAATTGGTAAGAGCACCG	45.0	47.6	55.8	57.5	1104-1120 bp	1.0	59.2
psbM-trnD-GUC_p4	AGAATGAACAGTGCAGTAGC	CAATTGGTAAGAGCACCGCC	45.0	55.0	55.8	59.5	1101-1117 bp	1.0	58.6
psbM-trnD-GUC_p5	AGAATGAACAGTGCAGTAGC	TTCAATTGGTAAGAGCACCG	45.0	45.0	55.8	56.1	1103-1119 bp	1.0	55.4