Hotspot

trnC-GCA-petN

LSC · 27844-28673 · 830 bp

Back to Rosularia

Pi 0.0984

MarkerSeek score 82.20

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 830
variable_sites: 81
pi: 0.0984
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	901-908 bp	1.0	87.1
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.1	57.8	895-902 bp	1.0	85.6
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.4	58.0	919-926 bp	1.0	84.7
trnC-GCA-petN_p4	GGGGGACTGCAAATCCTTTT	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.4	57.8	913-920 bp	1.0	83.3
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	CATTAAAGCAGCCCAAGCAA	50.0	45.0	58.1	57.3	903-910 bp	1.0	81.1