Hotspot

trnS-GCU-trnG-GCC

LSC · 8489-9104 · 616 bp

Back to Rosularia

Pi 0.116

MarkerSeek score 86.00

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 616
variable_sites: 71
pi: 0.116
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 86.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	CAATCCGACGCTTTAGTCCA	TGTTCGGAATACAAAGAAAACCG	50.0	39.1	58.0	57.9	740-774 bp	1.0	63.0
trnS-GCU-trnG-GCC_p2	AATCCGACGCTTTAGTCCAC	TGTTCGGAATACAAAGAAAACCG	50.0	39.1	58.0	57.9	739-773 bp	1.0	63.0
trnS-GCU-trnG-GCC_p3	CAATCCGACGCTTTAGTCCA	CGAAGGACCCCTTAACTATTAGG	50.0	47.8	58.0	57.8	719-753 bp	1.0	62.3
trnS-GCU-trnG-GCC_p4	AATCCGACGCTTTAGTCCAC	CGAAGGACCCCTTAACTATTAGG	50.0	47.8	58.0	57.8	718-752 bp	1.0	62.3
trnS-GCU-trnG-GCC_p5	GCTCGTACAACGGATTAGCA	TGTTCGGAATACAAAGAAAACCG	50.0	39.1	58.1	57.9	758-792 bp	1.0	62.2