Hotspot

psbI-trnS-GCU

LSC · 8233-8400 · 168 bp

Back to Rosularia

Pi 0.0932

MarkerSeek score 81.90

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 168
variable_sites: 15
pi: 0.0932
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	GGACGTAATCCTGGACGAGA	TGGACTAAAGCGTCGGATTG	55.0	50.0	58.6	58.0	268-274 bp	1.0	81.6
psbI-trnS-GCU_p2	GGACGTAATCCTGGACGAGA	GTGGACTAAAGCGTCGGATT	55.0	50.0	58.6	58.0	269-275 bp	1.0	81.5
psbI-trnS-GCU_p3	GGACGTAATCCTGGACGAGA	TGCTAATCCGTTGTACGAGC	55.0	50.0	58.6	58.1	250-256 bp	1.0	81.4
psbI-trnS-GCU_p4	GGACGTAATCCTGGACGAGA	ATCCGTTGTACGAGCGATTC	55.0	50.0	58.6	58.2	245-251 bp	1.0	80.9
psbI-trnS-GCU_p5	GGACGTAATCCTGGACGAGA	AATCCGTTGTACGAGCGATT	55.0	45.0	58.6	57.7	246-252 bp	1.0	79.8