Hotspot

rpl22

LSC · 81983-82375 · 393 bp

Back to Rostellularia

Pi 0.0331

MarkerSeek score 71.90

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 393
variable_sites: 13
pi: 0.0331
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	CAAGTCGGAAACCAAGTGGA	AAATTTCCGAGGACACGCAA	50.0	45.0	58.0	58.4	538-573 bp	1.0	78.4
rpl22_p2	CAAGTCGGAAACCAAGTGGA	AATTTCCGAGGACACGCAAA	50.0	45.0	58.0	58.4	537-572 bp	1.0	78.4
rpl22_p3	CAAGTCGGAAACCAAGTGGA	ATTTCCGAGGACACGCAAAA	50.0	45.0	58.0	58.4	536-571 bp	1.0	78.4
rpl22_p4	CAAGTCGGAAACCAAGTGGA	TAAATTTCCGAGGACACGCA	50.0	45.0	58.0	57.5	539-574 bp	1.0	77.9
rpl22_p5	GTGCAAACCAAAGCGAATGA	ATTTCCGAGGACACGCAAAA	45.0	45.0	57.9	58.4	572-607 bp	1.0	77.5