Hotspot

rps15-ycf1

SSC · 120062-120556 · 495 bp

Back to Rostellularia

Pi 0.0525

MarkerSeek score 82.90

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 495
variable_sites: 24
pi: 0.0525
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CCCCGCGTTTTCTTCTTTTT	GGATCCGCCTGTATCCACTA	45.0	55.0	57.5	58.4	593-605 bp	1.0	80.2
rps15-ycf1_p2	ACCCCGCGTTTTCTTCTTTT	GGATCCGCCTGTATCCACTA	45.0	55.0	58.6	58.4	594-606 bp	1.0	79.4
rps15-ycf1_p3	TGAAATTCAACAGACCCCGC	GGATCCGCCTGTATCCACTA	50.0	55.0	58.8	58.4	607-619 bp	1.0	78.1
rps15-ycf1_p4	GACCCCGCGTTTTCTTCTTT	GGATCCGCCTGTATCCACTA	50.0	55.0	59.1	58.4	595-607 bp	1.0	75.8
rps15-ycf1_p5	ACCCCGCGTTTTCTTCTTT	GGATCCGCCTGTATCCACTA	47.4	55.0	57.9	58.4	594-606 bp	1.0	75.4