Hotspot

ndhI-ndhA

SSC · 116392-116470 · 79 bp

Back to Rostellularia

Pi 0.0759

MarkerSeek score 80.00

Primer available yes

Label ndhI-ndhA

Feature Metadata

label_name: ndhI-ndhA
feature_type: igs
region: SSC
length_bp: 79
variable_sites: 6
pi: 0.0759
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhA_p1	TGGCTCGGATTGTTTGTTGA	ACCGAGACTAAGAATGGACCA	45.0	47.6	58.0	58.2	243 bp	1.0	72.3
ndhI-ndhA_p2	GTGGCTCGGATTGTTTGTTG	ACCGAGACTAAGAATGGACCA	50.0	47.6	57.9	58.2	244 bp	1.0	72.1
ndhI-ndhA_p3	GTATCTGGTGGCTCGGATTG	ACCGAGACTAAGAATGGACCA	55.0	47.6	58.1	58.2	251 bp	1.0	71.4
ndhI-ndhA_p4	ATGTATCTGGTGGCTCGGAT	ACCGAGACTAAGAATGGACCA	50.0	47.6	58.3	58.2	253 bp	1.0	70.2
ndhI-ndhA_p5	GCTCGGATTGTTTGTTGACC	ACCGAGACTAAGAATGGACCA	50.0	47.6	57.7	58.2	241 bp	1.0	69.9