Hotspot

ndhG-ndhI

SSC · 115580-115884 · 305 bp

Back to Rostellularia

Pi 0.0488

MarkerSeek score 70.80

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 305
variable_sites: 14
pi: 0.0488
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	GGATCCCATACCAGGACGTA	TCAACAAACAATCCGAGCCA	55.0	45.0	58.0	58.0	1169-1174 bp	1.0	82.5
ndhG-ndhI_p2	GGATCCCATACCAGGACGTA	ACCTACCCCTCGTAGATTGG	55.0	55.0	58.0	57.9	975-980 bp	1.0	82.5
ndhG-ndhI_p3	GGATCCCATACCAGGACGTA	CAACAAACAATCCGAGCCAC	55.0	50.0	58.0	57.9	1168-1173 bp	1.0	82.3
ndhG-ndhI_p4	CGCAGCAGCTACAAAATACG	TCAACAAACAATCCGAGCCA	50.0	45.0	57.9	58.0	982-987 bp	1.0	82.3
ndhG-ndhI_p5	CGCAGCAGCTACAAAATACG	CAACAAACAATCCGAGCCAC	50.0	50.0	57.9	57.9	981-986 bp	1.0	82.2