Hotspot

petA-psbJ

LSC · 62203-63131 · 929 bp

Back to Rostellularia

Pi 0.0221

MarkerSeek score 73.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 929
variable_sites: 20
pi: 0.0221
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	GCCGATACTACTGGAAGGAT	50.0	50.0	58.0	56.2	1148-1168 bp	1.0	68.2
petA-psbJ_p2	CATTACGTGTCCAAGGCCTT	GCCGATACTACTGGAAGGAT	50.0	50.0	58.2	56.2	1145-1165 bp	1.0	67.1
petA-psbJ_p3	ATCCATTACGTGTCCAAGGC	GCCGATACTACTGGAAGGATT	50.0	47.6	58.0	57.0	1148-1168 bp	1.0	66.3
petA-psbJ_p4	ATCCATTACGTGTCCAAGGC	GCCGATACTACTGGAAGGATTC	50.0	50.0	58.0	58.1	1148-1168 bp	1.0	65.9
petA-psbJ_p5	TTACGTGTCCAAGGCCTTTT	GCCGATACTACTGGAAGGAT	45.0	50.0	57.6	56.2	1143-1163 bp	1.0	65.8