Hotspot

ndhK-ndhC

LSC · 49066-49123 · 58 bp

Back to Rostellularia

Pi 0.0517

MarkerSeek score 73.20

Primer available yes

Label ndhK-ndhC

Feature Metadata

label_name: ndhK-ndhC
feature_type: igs
region: LSC
length_bp: 58
variable_sites: 3
pi: 0.0517
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK-ndhC_p1	ACCATAGAGAAGCGGCCATA	GTTTATGCATGGCGAAAGGG	50.0	50.0	58.3	57.8	217 bp	1.0	77.2
ndhK-ndhC_p2	ACCATAGAGAAGCGGCCATA	GGGGCATTGGAATGGTCTTA	50.0	50.0	58.3	57.5	199 bp	1.0	75.3
ndhK-ndhC_p3	AACGGAATTTTCGGCTGTTC	GTTTATGCATGGCGAAAGGG	45.0	50.0	57.3	57.8	148 bp	1.0	74.4
ndhK-ndhC_p4	CCATAGAGAAGCGGCCATAA	GTTTATGCATGGCGAAAGGG	50.0	50.0	57.2	57.8	216 bp	1.0	72.7
ndhK-ndhC_p5	GCGGCCATAAACTAGAGAGT	GTTTATGCATGGCGAAAGGG	50.0	50.0	57.1	57.8	206 bp	1.0	72.4