Hotspot

trnL-UAA-trnF-GAA

LSC · 46950-47167 · 218 bp

Back to Rostellularia

Pi 0.116

MarkerSeek score 80.90

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 218
variable_sites: 22
pi: 0.116
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AAGAGGAAAATCCGTCGACC	TCTACCAGCTGAGCTATCCC	50.0	55.0	57.6	58.0	282-297 bp	1.0	87.0
trnL-UAA-trnF-GAA_p2	GAGGAAAATCCGTCGACCTT	TCTACCAGCTGAGCTATCCC	50.0	55.0	57.6	58.0	280-295 bp	1.0	87.0
trnL-UAA-trnF-GAA_p3	AGAGGAAAATCCGTCGACCT	TCTACCAGCTGAGCTATCCC	50.0	55.0	58.4	58.0	281-296 bp	1.0	86.5
trnL-UAA-trnF-GAA_p4	GAGGAAAATCCGTCGACCTT	TTTGAACTGGTGACACGAGG	50.0	50.0	57.6	58.1	316-331 bp	1.0	86.0
trnL-UAA-trnF-GAA_p5	AAGAGGAAAATCCGTCGACC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.6	58.1	318-333 bp	1.0	85.9