Hotspot

rps4-trnT-UGU

LSC · 45043-45699 · 657 bp

Back to Rostellularia

Pi 0.0435

MarkerSeek score 77.60

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 657
variable_sites: 24
pi: 0.0435
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	AAGATCGCTTCCGGCTTTAG	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.1	57.9	711-816 bp	1.0	85.0
rps4-trnT-UGU_p2	CTAAGATCGCTTCCGGCTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.1	57.9	713-818 bp	1.0	85.0
rps4-trnT-UGU_p3	TTTCTAAGATCGCTTCCGGC	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.1	57.9	716-821 bp	1.0	84.9
rps4-trnT-UGU_p4	AAGATCGCTTCCGGCTTTAG	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.1	57.8	679-784 bp	1.0	84.2
rps4-trnT-UGU_p5	CTAAGATCGCTTCCGGCTTT	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.1	57.8	681-786 bp	1.0	84.2