Hotspot

ycf3-trnS-GGA

LSC · 43384-44066 · 683 bp

Back to Rostellularia

Pi 0.027

MarkerSeek score 65.20

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 683
variable_sites: 18
pi: 0.027
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CCATTTATTCGCGATCTAGGC	CCCTCGGTAAACAAAAGCCT	47.6	50.0	57.0	58.1	758-773 bp	1.0	66.9
ycf3-trnS-GGA_p2	CCATTTATTCGCGATCTAGGC	ACGGAAAGAGAGGGATTCGA	47.6	50.0	57.0	58.2	779-794 bp	1.0	66.4
ycf3-trnS-GGA_p3	CCATTTATTCGCGATCTAGGC	TTCCAATGCTACGCCTTGAA	47.6	45.0	57.0	57.8	729-744 bp	1.0	66.3
ycf3-trnS-GGA_p4	CCATTTATTCGCGATCTAGGC	ACCCTCGGTAAACAAAAGCC	47.6	50.0	57.0	58.4	759-774 bp	1.0	64.6
ycf3-trnS-GGA_p5	TCCATTTATTCGCGATCTAGGC	CCCTCGGTAAACAAAAGCCT	45.5	50.0	58.4	58.1	759-774 bp	1.0	63.6