Hotspot

trnG-GCC-trnfM-CAU

LSC · 35471-35596 · 126 bp

Back to Rostellularia

Pi 0.0424

MarkerSeek score 71.70

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 126
variable_sites: 5
pi: 0.0424
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	AGAAGATGCGGGTTCGATTC	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	58.1	58.0	228-232 bp	1.0	83.8
trnG-GCC-trnfM-CAU_p2	GAGAAGATGCGGGTTCGATT	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	58.1	58.0	229-233 bp	1.0	83.8
trnG-GCC-trnfM-CAU_p3	AGAAGATGCGGGTTCGATTC	GTTCAAATCCTGTCTCCGCA	50.0	50.0	58.1	58.2	179-183 bp	1.0	83.7
trnG-GCC-trnfM-CAU_p4	GAGAAGATGCGGGTTCGATT	GTTCAAATCCTGTCTCCGCA	50.0	50.0	58.1	58.2	180-184 bp	1.0	83.7
trnG-GCC-trnfM-CAU_p5	AGAAGATGCGGGTTCGATTC	TAGCTCGCAAGGCTCATAAC	50.0	50.0	58.1	57.8	212-216 bp	1.0	82.6