Hotspot

trnE-UUC-trnT-GGU

LSC · 29753-30485 · 733 bp

Back to Rostellularia

Pi 0.0861

MarkerSeek score 82.20

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 733
variable_sites: 51
pi: 0.0861
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	768-837 bp	1.0	87.7
trnE-UUC-trnT-GGU_p2	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	805-874 bp	1.0	87.5
trnE-UUC-trnT-GGU_p3	CGTTGCCTCCTTGAAAGAGA	CCGATGACTTACGCCTTACC	50.0	55.0	57.8	58.2	765-834 bp	1.0	86.5
trnE-UUC-trnT-GGU_p4	TTTCGTAGTACCCTACCCCC	CCGATGACTTACGCCTTACC	55.0	55.0	57.8	58.2	802-871 bp	1.0	86.4
trnE-UUC-trnT-GGU_p5	TTTCGTAGTACCCTACCCCC	TTGAACCGATGACTTACGCC	55.0	50.0	57.8	58.3	807-876 bp	1.0	85.3