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psbK-psbI

LSC · 7005-7458 · 454 bp

Back to Rostellularia

Pi 0.0467

MarkerSeek score 76.10

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 454
variable_sites: 21
pi: 0.0467
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CTTTTGTTTGGCAAGCTGCT	CGCCCCGGATCATTAGATAG	45.0	55.0	58.1	57.4	578-594 bp	1.0	78.3
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	CGCCCCGGATCATTAGATAG	50.0	55.0	57.9	57.4	573-589 bp	1.0	78.3
psbK-psbI_p3	GCCAGTCATACCTGTGCTTT	CGCCCCGGATCATTAGATAG	50.0	55.0	58.2	57.4	610-626 bp	1.0	77.2
psbK-psbI_p4	TGGCAAGCTGCTGTAAGTTT	CGCCCCGGATCATTAGATAG	45.0	55.0	58.3	57.4	570-586 bp	1.0	76.3
psbK-psbI_p5	TTGGCAAGCTGCTGTAAGTT	CGCCCCGGATCATTAGATAG	45.0	55.0	58.3	57.4	571-587 bp	1.0	76.3