Hotspot

rps16-trnQ-UUG

LSC · 5670-6390 · 721 bp

Back to Rostellularia

Pi 0.0526
MarkerSeek score 75.10
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
721
variable_sites
29
pi
0.0526
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.76
markerseek_score
75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 GCTTTCTGCCACATCGTTTC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 619-782 bp 1.0 86.5
rps16-trnQ-UUG_p2 AACGGATTCTGCCCTTCAAG GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 648-811 bp 1.0 85.2
rps16-trnQ-UUG_p3 CAACGGATTCTGCCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.5 58.1 649-812 bp 1.0 82.8
rps16-trnQ-UUG_p4 GCTTTCTGCCACATCGTTTC TTCGGAGGTTCGAATCCTTC 50.0 50.0 58.0 57.3 623-786 bp 1.0 81.7
rps16-trnQ-UUG_p5 GGATTCTGCCCTTCAAGTCG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.6 58.1 645-808 bp 1.0 81.6