Hotspot

ycf1

IRa · 132062-137512 · 5451 bp

Back to Roscoea

Pi 0.00322

MarkerSeek score 37.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRa
length_bp: 5451
variable_sites: 46
pi: 0.00322
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACATCAATAGTGTTTTTCGGGT	ACAGTGTATTTATGGCCGAATCA	36.4	39.1	56.3	58.2	299-305 bp	1.0	42.4
ycf1_p2	ACATCAATAGTGTTTTTCGGGT	ACAGTGTATTTATGGCCGAA	36.4	40.0	56.3	54.8	299-305 bp	1.0	42.4
ycf1_p3	ACATCAATAGTGTTTTTCGGGT	CAGTGTATTTATGGCCGAATCA	36.4	40.9	56.3	56.6	298-304 bp	1.0	42.4
ycf1_p4	ACATCAATAGTGTTTTTCGGGT	ACAGTGTATTTATGGCCGAATC	36.4	40.9	56.3	56.6	299-305 bp	1.0	42.4
ycf1_p5	ACATCAATAGTGTTTTTCGGGT	ACAGTGTATTTATGGCCGAAT	36.4	38.1	56.3	55.4	299-305 bp	1.0	42.4