Hotspot

rpl2-trnH-GUG

IRa · 162704-162751 · 48 bp

Back to Roscoea

Pi 0.0247

MarkerSeek score 62.10

Primer available yes

Label rpl2-trnH-GUG

Feature Metadata

label_name: rpl2-trnH-GUG
feature_type: igs
region: IRa
length_bp: 48
variable_sites: 4
pi: 0.0247
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-trnH-GUG_p1	GTTATCCTGCGCTTGGAAGA	TGGATCAAGGCAGTGGATTG	50.0	50.0	58.0	57.9	184-192 bp	1.0	83.0
rpl2-trnH-GUG_p2	GTTATCCTGCGCTTGGAAGA	GGATCAAGGCAGTGGATTGT	50.0	50.0	58.0	57.9	183-191 bp	1.0	83.0
rpl2-trnH-GUG_p3	GTTATCCTGCGCTTGGAAGA	GTGGATCAAGGCAGTGGATT	50.0	50.0	58.0	57.9	185-193 bp	1.0	83.0
rpl2-trnH-GUG_p4	GTTATCCTGCGCTTGGAAGA	CGGATGTAGCCAAGTGGATC	50.0	55.0	58.0	58.1	198-206 bp	1.0	82.8
rpl2-trnH-GUG_p5	CACAACCCCTTGGGGTTATC	CGGATGTAGCCAAGTGGATC	55.0	55.0	58.2	58.1	212-220 bp	1.0	81.5