Hotspot

ndhF-rpl32

SSC · 119833-120520 · 688 bp

Back to Roscoea

Pi 0.0102

MarkerSeek score 68.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 688
variable_sites: 18
pi: 0.0102
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CGGAATAAGTAGAAGCCCGA	AGAAAAAGCCTTTACTGCAGC	50.0	42.9	56.8	57.3	877-954 bp	1.0	63.0
ndhF-rpl32_p2	CGGAATAAGTAGAAGCCCGA	GAAAAAGCCTTTACTGCAGCT	50.0	42.9	56.8	57.3	876-953 bp	1.0	63.0
ndhF-rpl32_p3	TCGGAATAAGTAGAAGCCCG	AGAAAAAGCCTTTACTGCAGC	50.0	42.9	56.8	57.3	878-955 bp	1.0	63.0
ndhF-rpl32_p4	TCGGAATAAGTAGAAGCCCG	GAAAAAGCCTTTACTGCAGCT	50.0	42.9	56.8	57.3	877-954 bp	1.0	63.0
ndhF-rpl32_p5	GAAGCCCGAATCCTATTGACA	GAAAAAGCCTTTACTGCAGCT	47.6	42.9	57.8	57.3	865-942 bp	1.0	62.9