Hotspot

ycf1-ndhF

IRb · 116936-117615 · 680 bp

Back to Roscoea

Pi 0.0215

MarkerSeek score 59.70

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: IRb
length_bp: 680
variable_sites: 35
pi: 0.0215
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	GGGGAACGGAAACAGAACTT	TCCCAATGCGAGTTATTCCG	50.0	50.0	58.0	58.1	1709-1849 bp	1.0	85.4
ycf1-ndhF_p2	TCCGTTTAAAACCTTGGCGA	TCCCAATGCGAGTTATTCCG	45.0	50.0	58.0	58.1	1808-1948 bp	1.0	85.2
ycf1-ndhF_p3	TCGAAGGTCAATGCACAACT	TCCCAATGCGAGTTATTCCG	45.0	50.0	57.7	58.1	1125-1265 bp	1.0	84.5
ycf1-ndhF_p4	AACAGTTTGGGGAACGGAAA	TCCCAATGCGAGTTATTCCG	45.0	50.0	57.9	58.1	1717-1857 bp	1.0	84.4
ycf1-ndhF_p5	GGGGAACGGAAACAGAACTT	CAATGGGGTTGTTGTTGCAA	50.0	45.0	58.0	57.7	1385-1525 bp	1.0	83.4