Hotspot

psbM-trnD-GUC

LSC · 31242-32367 · 1126 bp

Back to Roscoea

Pi 0.00766

MarkerSeek score 63.50

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1126
variable_sites: 24
pi: 0.00766
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAGTGCAGTAGC	CCTCGCTGGGATTGTAGTTC	45.0	55.0	55.8	58.1	1214-1271 bp	1.0	67.2
psbM-trnD-GUC_p2	AGAATGAACAGTGCAGTAGC	TACACCTCGCTGGGATTGTA	45.0	50.0	55.8	58.1	1218-1275 bp	1.0	66.5
psbM-trnD-GUC_p3	AGAATGAACAGTGCAGTAGC	CATACACCTCGCTGGGATTG	45.0	55.0	55.8	58.4	1220-1277 bp	1.0	64.4
psbM-trnD-GUC_p4	AGGAACTAGAATGAACAGTGCA	CCTCGCTGGGATTGTAGTTC	40.9	55.0	57.4	58.1	1221-1278 bp	1.0	64.1
psbM-trnD-GUC_p5	AGAATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.8	58.5	1197-1254 bp	1.0	63.9