Hotspot

petN-psbM

LSC · 29836-31136 · 1301 bp

Back to Roscoea

Pi 0.00679

MarkerSeek score 65.90

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1301
variable_sites: 27
pi: 0.00679
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AGTATGGGGAAGGAGTGGAC	TGCTACTGCACTGTTCATTCT	55.0	42.9	58.1	57.6	1397-1467 bp	1.0	71.3
petN-psbM_p2	GTATGGGGAAGGAGTGGACT	TGCTACTGCACTGTTCATTCT	55.0	42.9	58.1	57.6	1396-1466 bp	1.0	71.3
petN-psbM_p3	ATGGGGAAGGAGTGGACTTT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1394-1464 bp	1.0	70.4
petN-psbM_p4	ATTGTAAGTCTCGCTTGGGC	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.3	57.6	1456-1526 bp	1.0	70.1
petN-psbM_p5	TGGGGAAGGAGTGGACTTTA	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	1393-1463 bp	1.0	68.9