Hotspot

rps16

LSC · 5246-6387 · 1142 bp

Back to Rosa

Pi 0.00943
MarkerSeek score 50.50
Primer available yes
Label rps16

Feature Metadata

label_name
rps16
feature_type
gene
region
LSC
length_bp
1142
variable_sites
66
pi
0.00943
unique_haplotype_count
46
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16_p1 AAGTCATCCACACCTTCTTT TGGGTTCCAAACTGAAGGAA 40.0 45.0 54.5 56.9 1186-1211 bp 0.413793 24.1
rps16_p2 AGTCATCCACACCTTCTTTT TGGGTTCCAAACTGAAGGAA 40.0 45.0 54.5 56.9 1185-1210 bp 0.413793 24.1
rps16_p3 AAGTCATCCACACCTTCTTTT TGGGTTCCAAACTGAAGGAA 38.1 45.0 55.3 56.9 1186-1211 bp 0.413793 22.9
rps16_p4 AGTCATCCACACCTTCTTTTT TGGGTTCCAAACTGAAGGAA 38.1 45.0 55.3 56.9 1185-1210 bp 0.413793 22.9
rps16_p5 AGTCATCCACACCTTCTTTT ATGGGTTCCAAACTGAAGGA 40.0 45.0 54.5 56.6 1186-1211 bp 0.413793 22.2