Hotspot

ndhF

SSC · 111739-113988 · 2250 bp

Back to Rosa

Pi 0.00486
MarkerSeek score 37.80
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2250
variable_sites
110
pi
0.00486
unique_haplotype_count
45
primer_available
yes
alignment_reliability
1.00
markerseek_score
37.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 AGAAGATCCTTCCCCTTCCC TGAGTCAAGTTATGGGCCCT 55.0 50.0 58.1 58.3 2901-3000 bp 0.436782 59.4
ndhF_p2 GAAGATCCTTCCCCTTCCCT TGAGTCAAGTTATGGGCCCT 55.0 50.0 58.1 58.3 2900-2999 bp 0.436782 59.4
ndhF_p3 GCAGAAGATCCTTCCCCTTC TGAGTCAAGTTATGGGCCCT 55.0 50.0 57.7 58.3 2903-3000 bp 0.425287 57.0
ndhF_p4 GAGGATCCGGACAAAATCGA AGCCGAACCAAACTGCAATA 50.0 45.0 57.4 58.1 2916-3000 bp 0.114943 42.8
ndhF_p5 GAGGATCCGGACAAAATCGA TAGCCGAACCAAACTGCAAT 50.0 45.0 57.4 58.1 2917-2999 bp 0.103448 42.1