Hotspot

ndhF-rpl32

SSC · 113989-115094 · 1106 bp

Back to Rosa

Pi 0.00799
MarkerSeek score 65.40
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1106
variable_sites
90
pi
0.00799
unique_haplotype_count
49
primer_available
yes
alignment_reliability
0.98
markerseek_score
65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CTGGAAGTGGAATGAACGGT AAAAGCTTTCAATGCTGCCC 50.0 45.0 57.8 57.8 1239-1340 bp 1.0 86.6
ndhF-rpl32_p2 CTGGAAGTGGAATGAACGGT ATGCTGCCCAATACCCTTTC 50.0 50.0 57.8 58.2 1228-1329 bp 1.0 86.3
ndhF-rpl32_p3 CTGGAAGTGGAATGAACGGT AATGCTGCCCAATACCCTTT 50.0 45.0 57.8 57.7 1229-1330 bp 1.0 86.0
ndhF-rpl32_p4 CTGGAAGTGGAATGAACGGT CAATGCTGCCCAATACCCTT 50.0 50.0 57.8 58.5 1230-1331 bp 0.965517 82.5
ndhF-rpl32_p5 CTGGAAGTGGAATGAACGGT TTCAATGCTGCCCAATACCC 50.0 50.0 57.8 58.5 1232-1333 bp 0.965517 82.4