Hotspot

psaJ-rpl33

LSC · 68301-68769 · 469 bp

Back to Rosa

Pi 0.0114
MarkerSeek score 61.00
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
469
variable_sites
32
pi
0.0114
unique_haplotype_count
40
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 TCCGGATGCGTTGATATTCC GGACATCTTTACCCTTGGCC 50.0 55.0 57.8 58.2 517-546 bp 1.0 85.7
psaJ-rpl33_p2 TCCGGATGCGTTGATATTCC TCTGGAAATGCCCGTTGATT 50.0 45.0 57.8 57.8 591-620 bp 1.0 85.4
psaJ-rpl33_p3 GTTCGGGTCTTTAGCAGGTT GGACATCTTTACCCTTGGCC 50.0 55.0 57.8 58.2 562-591 bp 1.0 85.4
psaJ-rpl33_p4 GTTCGGGTCTTTAGCAGGTT TCTGGAAATGCCCGTTGATT 50.0 45.0 57.8 57.8 636-665 bp 1.0 85.2
psaJ-rpl33_p5 TGGCACCGGTAGTAAGTACT TCTGGAAATGCCCGTTGATT 50.0 45.0 57.8 57.8 661-690 bp 1.0 84.7