Hotspot

accD-psaI

LSC · 60103-60545 · 443 bp

Back to Rosa

Pi 0.0117
MarkerSeek score 61.20
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
443
variable_sites
36
pi
0.0117
unique_haplotype_count
34
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 AAAGGGCGTTCTGAGTGAAT CCATTGCAATTGCCGGAAAT 45.0 45.0 57.4 58.0 570-634 bp 1.0 84.3
accD-psaI_p2 AAGGGCGTTCTGAGTGAATT CCATTGCAATTGCCGGAAAT 45.0 45.0 57.4 58.0 569-633 bp 1.0 84.3
accD-psaI_p3 AAAGGGCGTTCTGAGTGAAT AGAAACCATTGCAATTGCCG 45.0 45.0 57.4 57.9 575-639 bp 1.0 83.8
accD-psaI_p4 AAGGGCGTTCTGAGTGAATT AGAAACCATTGCAATTGCCG 45.0 45.0 57.4 57.9 574-638 bp 1.0 83.8
accD-psaI_p5 AAGGGCGTTCTGAGTGAATT AGGCACAAAAATGGAGGGAA 45.0 45.0 57.4 57.6 535-599 bp 1.0 81.7