Hotspot

rpoB-trnC-GCA

LSC · 26713-27930 · 1218 bp

Back to Rosa

Pi 0.0108
MarkerSeek score 50.70
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1218
variable_sites
89
pi
0.0108
unique_haplotype_count
43
primer_available
yes
alignment_reliability
0.99
markerseek_score
50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 CGAGCCCATATCCTTGCTTT AATCGGAGGACGCCCTATTA 50.0 50.0 58.0 58.0 2141-2182 bp 1.0 88.2
rpoB-trnC-GCA_p2 CCGCCTACACAAGCAAATTG AATCGGAGGACGCCCTATTA 50.0 50.0 58.0 58.0 2333-2374 bp 1.0 87.6
rpoB-trnC-GCA_p3 TCCGCCTACACAAGCAAATT AATCGGAGGACGCCCTATTA 45.0 50.0 58.1 58.0 2334-2375 bp 1.0 87.0
rpoB-trnC-GCA_p4 CCGCCTACACAAGCAAATTG CCGGATTTGAACTGGGGAAA 50.0 50.0 58.0 58.1 1950-1991 bp 1.0 86.0
rpoB-trnC-GCA_p5 CCGCCTACACAAGCAAATTG TCCGTCCTGCTATCCAATCT 50.0 50.0 58.0 57.9 2125-2164 bp 0.988506 85.3