Hotspot

trnR-UCU-atpA

LSC · 10106-10633 · 528 bp

Back to Rosa

Pi 0.00996
MarkerSeek score 51.50
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
528
variable_sites
45
pi
0.00996
unique_haplotype_count
43
primer_available
yes
alignment_reliability
0.97
markerseek_score
51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 ATTGGAGTGAAAAGCGTCCA TGGAACGTTTTCTACTTCAGGA 45.0 40.9 57.7 57.3 645-763 bp 1.0 66.0
trnR-UCU-atpA_p2 TGGAGTGAAAAGCGTCCATT TGGAACGTTTTCTACTTCAGGA 45.0 40.9 57.7 57.3 643-761 bp 1.0 66.0
trnR-UCU-atpA_p3 TTGGAGTGAAAAGCGTCCAT TGGAACGTTTTCTACTTCAGGA 45.0 40.9 57.7 57.3 644-762 bp 1.0 66.0
trnR-UCU-atpA_p4 GGAGTGAAAAGCGTCCATTG TGGAACGTTTTCTACTTCAGGA 50.0 40.9 57.4 57.3 642-760 bp 1.0 63.2
trnR-UCU-atpA_p5 TGGAGTGAAAAGCGTCCATT TGGAACGTTTTCTACTTCAGGAA 45.0 39.1 57.7 58.0 643-761 bp 1.0 63.2