Hotspot

trnK-UUU-rps16

LSC · 4295-5245 · 951 bp

Back to Rosa

Pi 0.00937
MarkerSeek score 47.60
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
951
variable_sites
77
pi
0.00937
unique_haplotype_count
56
primer_available
yes
alignment_reliability
0.82
markerseek_score
47.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCTTTCAGGATCAGTCGTGG AAAAAGGCGCTCAACCTACA 55.0 45.0 58.0 58.0 912-1213 bp 1.0 90.2
trnK-UUU-rps16_p2 CCTTTCAGGATCAGTCGTGG CGACGAGAGGGAAGAGATCT 55.0 55.0 58.0 58.0 1006-1307 bp 1.0 89.7
trnK-UUU-rps16_p3 GACTCTCCAGAACCTCCCTT AAAAAGGCGCTCAACCTACA 55.0 45.0 58.1 58.0 1404-1705 bp 0.850575 82.8
trnK-UUU-rps16_p4 CCTTTCAGGATCAGTCGTGG TTTTCTCGAGCCGTACGAAG 55.0 50.0 58.0 58.0 1376-1380 bp 0.057471 42.7
trnK-UUU-rps16_p5 GACTCTCCAGAACCTCCCTT TTTTCTCGAGCCGTACGAAG 55.0 50.0 58.1 58.0 1869-1874 bp 0.057471 42.4