primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.161600	79	391	398-476	1.000000	90.7
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.165962	79	391	412-490	1.000000	90.6
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.167469	79	391	411-489	1.000000	90.6
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.167469	79	391	413-491	1.000000	90.6
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	20	20	55.0	55.0	58.1	57.9	0.0	13.4	0.194818	79	391	348-426	1.000000	90.4
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CCTTTCAGGATCAGTCGTGG	AAAAAGGCGCTCAACCTACA	20	20	55.0	45.0	58.0	58.0	0.0	7.6	0.040076	4295	5245	912-1213	1.000000	90.2
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	CCTTTCAGGATCAGTCGTGG	CGACGAGAGGGAAGAGATCT	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.053942	4295	5245	1006-1307	1.000000	89.7
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	GACTCTCCAGAACCTCCCTT	AAAAAGGCGCTCAACCTACA	20	20	55.0	45.0	58.1	58.0	0.0	7.6	0.078760	4295	5245	1404-1705	0.850575	82.8
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CCTTTCAGGATCAGTCGTGG	TTTTCTCGAGCCGTACGAAG	20	20	55.0	50.0	58.0	58.0	0.0	18.8	0.042598	4295	5245	1376-1380	0.057471	42.7
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	GACTCTCCAGAACCTCCCTT	TTTTCTCGAGCCGTACGAAG	20	20	55.0	50.0	58.1	58.0	0.0	18.8	0.081282	4295	5245	1869-1874	0.057471	42.4
rps16_p1	rps16	1	AAGTCATCCACACCTTCTTT	TGGGTTCCAAACTGAAGGAA	20	20	40.0	45.0	54.5	56.9	0.0	0.0	4.676064	5246	6387	1186-1211	0.413793	24.1
rps16_p2	rps16	2	AGTCATCCACACCTTCTTTT	TGGGTTCCAAACTGAAGGAA	20	20	40.0	45.0	54.5	56.9	0.0	0.0	4.676064	5246	6387	1185-1210	0.413793	24.1
rps16_p3	rps16	3	AAGTCATCCACACCTTCTTTT	TGGGTTCCAAACTGAAGGAA	21	20	38.1	45.0	55.3	56.9	0.0	0.0	4.819252	5246	6387	1186-1211	0.413793	22.9
rps16_p4	rps16	4	AGTCATCCACACCTTCTTTTT	TGGGTTCCAAACTGAAGGAA	21	20	38.1	45.0	55.3	56.9	0.0	0.0	4.819252	5246	6387	1185-1210	0.413793	22.9
rps16_p5	rps16	5	AGTCATCCACACCTTCTTTT	ATGGGTTCCAAACTGAAGGA	20	20	40.0	45.0	54.5	56.6	0.0	0.0	4.911084	5246	6387	1186-1211	0.413793	22.2
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6388	7252	883-938	1.000000	90.3
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AAGTCGCACGTTGCTTTCTA	GCTACTCGGAGGTTCGAATC	20	20	45.0	55.0	58.1	57.9	8.1	10.2	0.261887	6388	7252	891-946	1.000000	89.9
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	6388	7252	894-949	0.988506	89.3
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	20	20	45.0	55.0	58.1	57.8	8.1	16.9	0.350677	6388	7252	887-942	1.000000	89.2
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	TCATGTCCTTCAAGTCGCAC	GCTACTCGGAGGTTCGAATC	20	20	50.0	55.0	58.2	57.9	0.0	10.2	0.326795	6388	7252	902-957	0.988506	88.9
trnR-UCU-atpA_p1	trnR-UCU-atpA	1	ATTGGAGTGAAAAGCGTCCA	TGGAACGTTTTCTACTTCAGGA	20	22	45.0	40.9	57.7	57.3	0.0	0.5	2.946328	10106	10633	645-763	1.000000	66.0
trnR-UCU-atpA_p2	trnR-UCU-atpA	2	TGGAGTGAAAAGCGTCCATT	TGGAACGTTTTCTACTTCAGGA	20	22	45.0	40.9	57.7	57.3	0.0	0.5	2.946328	10106	10633	643-761	1.000000	66.0
trnR-UCU-atpA_p3	trnR-UCU-atpA	3	TTGGAGTGAAAAGCGTCCAT	TGGAACGTTTTCTACTTCAGGA	20	22	45.0	40.9	57.7	57.3	0.0	0.5	2.946328	10106	10633	644-762	1.000000	66.0
trnR-UCU-atpA_p4	trnR-UCU-atpA	4	GGAGTGAAAAGCGTCCATTG	TGGAACGTTTTCTACTTCAGGA	20	22	50.0	40.9	57.4	57.3	0.0	0.5	3.294989	10106	10633	642-760	1.000000	63.2
trnR-UCU-atpA_p5	trnR-UCU-atpA	5	TGGAGTGAAAAGCGTCCATT	TGGAACGTTTTCTACTTCAGGAA	20	23	45.0	39.1	57.7	58.0	0.0	0.5	3.296390	10106	10633	643-761	1.000000	63.2
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	GCGAATCCATGGAAGGTCAT	20	20	45.0	50.0	58.0	58.0	1.4	20.7	0.065515	13485	14412	813-1006	1.000000	87.7
atpH-atpI_p2	atpH-atpI	2	TACCTTGACCAACTCCAGGT	GCGAATCCATGGAAGGTCAT	20	20	50.0	50.0	57.9	58.0	0.0	20.7	0.139407	13485	14412	869-1062	1.000000	87.0
atpH-atpI_p3	atpH-atpI	3	AATAACGGAAGCGGCAGAAA	GCGAATCCATGGAAGGTCAT	20	20	45.0	50.0	58.2	58.0	1.4	20.7	0.211053	13485	14412	814-1007	1.000000	86.5
atpH-atpI_p4	atpH-atpI	4	GCCAATCCAGCAGCAATAAC	GCGAATCCATGGAAGGTCAT	20	20	50.0	50.0	57.8	58.0	0.0	20.7	0.241515	13485	14412	828-1021	1.000000	86.2
atpH-atpI_p5	atpH-atpI	5	GCAGTACCTTGACCAACTCC	GCGAATCCATGGAAGGTCAT	20	20	55.0	50.0	58.2	58.0	0.0	20.7	0.217444	13485	14412	873-1063	0.965517	84.9
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	CGAGCCCATATCCTTGCTTT	AATCGGAGGACGCCCTATTA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.032498	26713	27930	2141-2182	1.000000	88.2
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	CCGCCTACACAAGCAAATTG	AATCGGAGGACGCCCTATTA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.024117	26713	27930	2333-2374	1.000000	87.6
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	TCCGCCTACACAAGCAAATT	AATCGGAGGACGCCCTATTA	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.101083	26713	27930	2334-2375	1.000000	87.0
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	CCGCCTACACAAGCAAATTG	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.100874	26713	27930	1950-1991	1.000000	86.0
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	CCGCCTACACAAGCAAATTG	TCCGTCCTGCTATCCAATCT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.106851	26713	27930	2125-2164	0.988506	85.3
petN-psbM_p1	petN-psbM	1	AGTAAATCTCGCTTGGGCTG	AAAACAGTCAGCCAAGGTGA	20	20	50.0	45.0	58.0	57.6	0.0	0.0	0.465211	28876	30157	1286-1410	1.000000	85.6
petN-psbM_p2	petN-psbM	2	AGTAAATCTCGCTTGGGCTG	AAACAGTCAGCCAAGGTGAT	20	20	50.0	45.0	58.0	57.3	0.0	0.0	0.694240	28876	30157	1285-1409	1.000000	83.7
petN-psbM_p3	petN-psbM	3	AGTAAATCTCGCTTGGGCTG	AACAGTCAGCCAAGGTGATT	20	20	50.0	45.0	58.0	57.3	0.0	0.0	0.694240	28876	30157	1284-1408	1.000000	83.7
petN-psbM_p4	petN-psbM	4	ATGGGGAAGAAGTGGACTCT	AAAACAGTCAGCCAAGGTGA	20	20	50.0	45.0	57.6	57.6	0.0	0.0	0.786235	28876	30157	1226-1350	1.000000	83.2
petN-psbM_p5	petN-psbM	5	ATGGGGAAGAAGTGGACTCT	AACAGTCAGCCAAGGTGATT	20	20	50.0	45.0	57.6	57.3	0.0	0.0	1.015264	28876	30157	1224-1348	1.000000	81.3
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	TTCTCCTGAAGTTGTCGGAA	ACGGAAAGAGAGGGATTCGA	20	20	45.0	50.0	56.4	58.2	0.0	0.0	1.735519	45099	45985	1057-1077	1.000000	73.6
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	TTTCTCCTGAAGTTGTCGGA	ACGGAAAGAGAGGGATTCGA	20	20	45.0	50.0	56.4	58.2	0.0	0.0	1.735519	45099	45985	1058-1078	1.000000	73.6
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	TTCTCCTGAAGTTGTCGGAA	TTCCAATGCTACGCCTTCAA	20	20	45.0	45.0	56.4	57.8	0.0	0.0	1.776834	45099	45985	1007-1027	1.000000	73.6
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	TTTCTCCTGAAGTTGTCGGA	TTCCAATGCTACGCCTTCAA	20	20	45.0	45.0	56.4	57.8	0.0	0.0	1.776834	45099	45985	1008-1028	1.000000	73.6
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	TTCTCCTGAAGTTGTCGGAA	AGTTCCAATGCTACGCCTTC	20	20	45.0	50.0	56.4	58.3	0.0	0.0	1.845104	45099	45985	1009-1029	1.000000	73.0
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	47436	48615	1056-1272	1.000000	90.9
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	47436	48615	1064-1280	1.000000	90.4
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	47436	48615	1088-1304	1.000000	90.0
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	ATGGAGAAAAGCCGGCTATC	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.7	58.0	33.1	0.0	0.267047	47436	48615	1108-1324	1.000000	89.9
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	47436	48615	1096-1312	1.000000	89.5
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.9	57.8	5.2	0.0	0.336606	52130	52737	731-822	1.000000	85.4
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	GGGGCTAAAACTCCGGAAAT	GCACGTTGGGTCTTTGAAAC	20	20	50.0	50.0	57.9	58.2	5.2	0.0	0.304202	52130	52737	795-886	1.000000	85.1
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TTCTGGCCCTTTGCTAATCG	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	58.2	57.8	0.0	0.0	0.447090	52130	52737	750-841	1.000000	84.5
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TTCTGGCCCTTTGCTAATCG	GCACGTTGGGTCTTTGAAAC	20	20	50.0	50.0	58.2	58.2	0.0	0.0	0.414686	52130	52737	814-905	1.000000	84.3
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	CCTTTGCTAATCGGGGCTAA	GCACGTTGGGTCTTTGAAAC	20	20	50.0	50.0	57.7	58.2	0.0	0.0	0.506725	52130	52737	807-898	1.000000	83.5
accD-psaI_p1	accD-psaI	1	AAAGGGCGTTCTGAGTGAAT	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	57.4	58.0	0.0	26.9	0.595626	60103	60545	570-634	1.000000	84.3
accD-psaI_p2	accD-psaI	2	AAGGGCGTTCTGAGTGAATT	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	57.4	58.0	0.0	26.9	0.595626	60103	60545	569-633	1.000000	84.3
accD-psaI_p3	accD-psaI	3	AAAGGGCGTTCTGAGTGAAT	AGAAACCATTGCAATTGCCG	20	20	45.0	45.0	57.4	57.9	0.0	26.9	0.651789	60103	60545	575-639	1.000000	83.8
accD-psaI_p4	accD-psaI	4	AAGGGCGTTCTGAGTGAATT	AGAAACCATTGCAATTGCCG	20	20	45.0	45.0	57.4	57.9	0.0	26.9	0.651789	60103	60545	574-638	1.000000	83.8
accD-psaI_p5	accD-psaI	5	AAGGGCGTTCTGAGTGAATT	AGGCACAAAAATGGAGGGAA	20	20	45.0	45.0	57.4	57.6	0.0	0.0	0.965667	60103	60545	535-599	1.000000	81.7
petA-psbJ_p1	petA-psbJ	1	ATCCATTACGTGTCCAAGGC	GCCGATACTACTGGAAGGAT	20	20	50.0	50.0	58.0	56.2	0.0	0.0	1.831892	63953	64914	1181-1233	0.988506	73.9
petA-psbJ_p2	petA-psbJ	2	CATTACGTGTCCAAGGCCTT	GCCGATACTACTGGAAGGAT	20	20	50.0	50.0	58.2	56.2	13.8	0.0	1.972289	63953	64914	1178-1230	0.988506	72.8
petA-psbJ_p3	petA-psbJ	3	ATCCATTACGTGTCCAAGGC	GCCGATACTACTGGAAGGATT	20	21	50.0	47.6	58.0	57.0	0.0	0.0	2.069983	63953	64914	1181-1233	0.988506	72.0
petA-psbJ_p4	petA-psbJ	4	ATCCATTACGTGTCCAAGGC	CCGGATTAGGTTCATCTCTGT	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.144234	63953	64914	1084-1136	0.988506	71.7
petA-psbJ_p5	petA-psbJ	5	ATCCATTACGTGTCCAAGGC	GCCGATACTACTGGAAGGATTC	20	22	50.0	50.0	58.0	58.1	0.0	0.0	2.122851	63953	64914	1181-1233	0.988506	71.5
psaJ-rpl33_p1	psaJ-rpl33	1	TCCGGATGCGTTGATATTCC	GGACATCTTTACCCTTGGCC	20	20	50.0	55.0	57.8	58.2	0.0	0.0	0.399850	68301	68769	517-546	1.000000	85.7
psaJ-rpl33_p2	psaJ-rpl33	2	TCCGGATGCGTTGATATTCC	TCTGGAAATGCCCGTTGATT	20	20	50.0	45.0	57.8	57.8	0.0	0.0	0.378194	68301	68769	591-620	1.000000	85.4
psaJ-rpl33_p3	psaJ-rpl33	3	GTTCGGGTCTTTAGCAGGTT	GGACATCTTTACCCTTGGCC	20	20	50.0	55.0	57.8	58.2	0.0	0.0	0.416464	68301	68769	562-591	1.000000	85.4
psaJ-rpl33_p4	psaJ-rpl33	4	GTTCGGGTCTTTAGCAGGTT	TCTGGAAATGCCCGTTGATT	20	20	50.0	45.0	57.8	57.8	0.0	0.0	0.394808	68301	68769	636-665	1.000000	85.2
psaJ-rpl33_p5	psaJ-rpl33	5	TGGCACCGGTAGTAAGTACT	TCTGGAAATGCCCGTTGATT	20	20	50.0	45.0	57.8	57.8	6.9	0.0	0.435029	68301	68769	661-690	1.000000	84.7
ndhF_p1	ndhF	1	AGAAGATCCTTCCCCTTCCC	TGAGTCAAGTTATGGGCCCT	20	20	55.0	50.0	58.1	58.3	3.3	25.7	0.430920	111739	113988	2901-3000	0.436782	59.4
ndhF_p2	ndhF	2	GAAGATCCTTCCCCTTCCCT	TGAGTCAAGTTATGGGCCCT	20	20	55.0	50.0	58.1	58.3	0.0	25.7	0.430920	111739	113988	2900-2999	0.436782	59.4
ndhF_p3	ndhF	3	GCAGAAGATCCTTCCCCTTC	TGAGTCAAGTTATGGGCCCT	20	20	55.0	50.0	57.7	58.3	3.3	25.7	0.671385	111739	113988	2903-3000	0.425287	57.0
ndhF_p4	ndhF	4	GAGGATCCGGACAAAATCGA	AGCCGAACCAAACTGCAATA	20	20	50.0	45.0	57.4	58.1	6.1	0.0	0.692617	111739	113988	2916-3000	0.114943	42.8
ndhF_p5	ndhF	5	GAGGATCCGGACAAAATCGA	TAGCCGAACCAAACTGCAAT	20	20	50.0	45.0	57.4	58.1	6.1	0.0	0.692617	111739	113988	2917-2999	0.103448	42.1
ndhF-rpl32_p1	ndhF-rpl32	1	CTGGAAGTGGAATGAACGGT	AAAAGCTTTCAATGCTGCCC	20	20	50.0	45.0	57.8	57.8	0.0	13.1	0.359368	113989	115094	1239-1340	1.000000	86.6
ndhF-rpl32_p2	ndhF-rpl32	2	CTGGAAGTGGAATGAACGGT	ATGCTGCCCAATACCCTTTC	20	20	50.0	50.0	57.8	58.2	0.0	0.0	0.404502	113989	115094	1228-1329	1.000000	86.3
ndhF-rpl32_p3	ndhF-rpl32	3	CTGGAAGTGGAATGAACGGT	AATGCTGCCCAATACCCTTT	20	20	50.0	45.0	57.8	57.7	0.0	0.0	0.437539	113989	115094	1229-1330	1.000000	86.0
ndhF-rpl32_p4	ndhF-rpl32	4	CTGGAAGTGGAATGAACGGT	CAATGCTGCCCAATACCCTT	20	20	50.0	50.0	57.8	58.5	0.0	0.0	0.696614	113989	115094	1230-1331	0.965517	82.5
ndhF-rpl32_p5	ndhF-rpl32	5	CTGGAAGTGGAATGAACGGT	TTCAATGCTGCCCAATACCC	20	20	50.0	50.0	57.8	58.5	0.0	0.0	0.700966	113989	115094	1232-1333	0.965517	82.4
ndhA_p1	ndhA	1	TGACCATAATTCATGAACCCAGT	CCGCCGGGTTTTATCAATTT	23	20	39.1	45.0	57.8	57.0	11.1	2.2	4.143185	121606	123953	2532-2619	1.000000	54.7
ndhA_p2	ndhA	2	TGACCATAATTCATGAACCCAGT	GCCGGGTTTTATCAATTTGCA	23	21	39.1	42.9	57.8	58.0	11.1	2.2	4.225677	121606	123953	2530-2617	1.000000	54.0
ndhA_p3	ndhA	3	TGACCATAATTCATGAACCCAGT	CCGCCGGGTTTTATCAATTTG	23	21	39.1	47.6	57.8	58.5	11.1	2.2	4.656397	121606	123953	2532-2619	1.000000	50.6
ndhA_p4	ndhA	4	TCATGAACCCAGTTACCATAGA	CCGCCGGGTTTTATCAATTT	22	20	40.9	45.0	56.3	57.0	0.0	2.2	4.690759	121606	123953	2522-2609	1.000000	50.3
ndhA_p5	ndhA	5	TGACCATAATTCATGAACCCAGT	TGGGAGAAGTTGATCGTTGA	23	20	39.1	45.0	57.8	56.5	11.1	0.0	4.704332	121606	123953	2435-2522	1.000000	50.3
ycf1_p1	ycf1	1	TGTGGTACTAGTCTAGACCATCT	TATCCCACCTTTCACAAGCC	23	20	43.5	50.0	57.3	57.5	23.0	0.0	4.219490	127764	127883	280-280	1.000000	53.1
ycf1_p2	ycf1	2	TGTGGTACTAGTCTAGACCATCT	CCCACCTTTCACAAGCCTAT	23	20	43.5	50.0	57.3	57.5	23.0	0.0	4.226542	127764	127883	277-277	1.000000	53.1
ycf1_p3	ycf1	3	TGTGGTACTAGTCTAGACCATCT	TCCCACCTTTCACAAGCCTA	23	20	43.5	50.0	57.3	58.6	23.0	0.0	4.271466	127764	127883	278-278	1.000000	52.7
ycf1_p4	ycf1	4	TGTGGTACTAGTCTAGACCA	TATCCCACCTTTCACAAGCC	20	20	45.0	50.0	53.9	57.5	19.4	0.0	4.585773	127764	127883	280-280	1.000000	50.2
ycf1_p5	ycf1	5	TGTGGTACTAGTCTAGACCA	CCCACCTTTCACAAGCCTAT	20	20	45.0	50.0	53.9	57.5	19.4	0.0	4.592824	127764	127883	277-277	1.000000	50.2