Hotspot

ccsA-ndhD

SSC · 142792-143012 · 221 bp

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Pi 0.0318

MarkerSeek score 67.60

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 221
variable_sites: 11
pi: 0.0318
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TCTGCAATTGTGGCTTCGAT	AGCAAGTGATAAGGTCGAATCC	45.0	45.5	58.2	58.2	360-378 bp	1.0	67.2
ccsA-ndhD_p2	TCTGCAATTGTGGCTTCGAT	GCAAGTGATAAGGTCGAATCC	45.0	47.6	58.2	56.8	359-377 bp	1.0	67.0
ccsA-ndhD_p3	TTCTGCAATTGTGGCTTCGA	AGCAAGTGATAAGGTCGAATCC	45.0	45.5	58.4	58.2	361-379 bp	1.0	65.4
ccsA-ndhD_p4	TTCTGCAATTGTGGCTTCGA	GCAAGTGATAAGGTCGAATCC	45.0	47.6	58.4	56.8	360-378 bp	1.0	65.3
ccsA-ndhD_p5	TCTGCAATTGTGGCTTCGAT	GCAAGTGATAAGGTCGAATCCA	45.0	45.5	58.2	58.5	359-377 bp	1.0	65.1