Hotspot

rpl32-trnL-UAG

SSC · 140692-141611 · 920 bp

Back to Rorippa

Pi 0.0375

MarkerSeek score 79.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 920
variable_sites: 56
pi: 0.0375
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TGGACATCGTTGAAAGCTTT	TCCTAAGAGCAGCGTGTCTA	40.0	50.0	55.9	57.9	999-1043 bp	1.0	70.4
rpl32-trnL-UAG_p2	TGGACATCGTTGAAAGCTTT	AGCAGCGTGTCTACCAATTT	40.0	45.0	55.9	57.8	992-1036 bp	1.0	70.0
rpl32-trnL-UAG_p3	TGGACATCGTTGAAAGCTTT	CCTAAGAGCAGCGTGTCTAC	40.0	55.0	55.9	57.8	998-1042 bp	1.0	69.8
rpl32-trnL-UAG_p4	TGGACATCGTTGAAAGCTTT	CTAAGAGCAGCGTGTCTACC	40.0	55.0	55.9	57.8	997-1041 bp	1.0	69.8
rpl32-trnL-UAG_p5	TGGACATCGTTGAAAGCTTT	GAGCAGCGTGTCTACCAATT	40.0	50.0	55.9	58.3	993-1037 bp	1.0	69.2