Hotspot

petA-psbJ

LSC · 89220-90233 · 1014 bp

Back to Rorippa

Pi 0.0222

MarkerSeek score 65.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1014
variable_sites: 39
pi: 0.0222
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	CAGGATTAGGTTCATCCCTGT	50.0	47.6	58.0	56.8	1135-1163 bp	1.0	66.7
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	TCAGGATTAGGTTCATCCCTGT	50.0	45.5	58.0	58.3	1136-1164 bp	1.0	66.4
petA-psbJ_p3	CATTACGTGTCCAAGGCCTT	CAGGATTAGGTTCATCCCTGT	50.0	47.6	58.2	56.8	1132-1160 bp	1.0	65.6
petA-psbJ_p4	CATTACGTGTCCAAGGCCTT	TCAGGATTAGGTTCATCCCTGT	50.0	45.5	58.2	58.3	1133-1161 bp	1.0	65.3
petA-psbJ_p5	ATCCATTACGTGTCCAAGGC	TCAGGATTAGGTTCATCCCTG	50.0	47.6	58.0	56.5	1136-1164 bp	1.0	64.5